二 、接種:就jiu是shi將jiang固gu體ti菌jun種zhong或huo液ye體ti菌jun種zhong接jie入ru菌jun棒bang的de過guo程cheng或huo方fang法fa。接jie種zhong的de整zheng個ge過guo程cheng都dou應ying按an無wu菌jun操cao作zuo要yao求qiu進jin行xing。能neng否fou做zuo到dao無wu菌jun操cao作zuo是shi接jie種zhong成cheng敗bai的de關guan鍵jian。接jie種zhong常chang用yong的de方fang法fa為wei接jie種zhong箱xiang和he接jie種zhong室shi接jie種zhong。
2.1 接種箱接種:jiezhongxiangjiezhongshiwoguoguangdanongcunyijiayihuzaipeishiyongjunzuichangyongdejiezhongfangfa。qiquedianshiyicijiezhongshuliangxiao,caozuobubian。yongjiezhongxiangjiezhongqiyuanlihefangfatongjiezhongshidatongxiaoyi,danyouruxiajidianqingzhuyi:①接種箱密閉性一定要好。接種箱的套袖要用不易透氣的二層布料做成。套袖前後要帶有二個鬆緊帶。兩個鬆緊帶之間距離為:18cm左右。②對於舊接種箱要先用水衝洗曬幹再進行一次熏蒸消毒,熏蒸用藥量是常規二倍。新接種箱也應先熏蒸一次。③接種箱盡量放在幹燥、幹淨、密閉性好的房間內。也可將接種箱放入塑料大棚內接種,但塑料大棚一定要幹燥,同時要求大棚提前晾曬並熏蒸消毒一次。
2.2 接種室接種:這(zhe)種(zhong)接(jie)種(zhong)方(fang)法(fa)是(shi)有(you)條(tiao)件(jian)的(de)菇(gu)場(chang)規(gui)模(mo)化(hua)生(sheng)產(chan)菌(jun)棒(bang)最(zui)常(chang)用(yong)的(de)一(yi)種(zhong)接(jie)種(zhong)方(fang)法(fa),此(ci)方(fang)法(fa)由(you)於(yu)操(cao)作(zuo)方(fang)便(bian),接(jie)種(zhong)速(su)度(du)快(kuai),有(you)取(qu)代(dai)利(li)用(yong)接(jie)種(zhong)箱(xiang)傳(chuan)統(tong)接(jie)種(zhong)方(fang)法(fa)的(de)趨(qu)勢(shi)。
2.2.1 對接種室的要求:要求接種室有良好的密閉性和通風性能,地麵、牆壁及天花板光滑便於消毒,接種室周圍環境要求清潔幹淨,接種室最好有紫外線燈和緩衝間。
2.2.2 接種室使用前的處理:xinjiandefangjianzuoweijiezhongshi,zaishiyongqianjiaqiangtongfengbingfangzhiyixieshengshihuikuaixishuishizhiganzao,tongshiyongdunbujiangdimiancaxiganjing。jiujiezhongshizhongxinshiyongshi,yingxianjiangqidasaocaxiganjinghou,zaiyongjiaquanhegaomengsuanjiaanmeilimi10毫升甲醛5g高錳酸鉀進行熏蒸。
2.2.3 菌種挑選與處理:菌jun種zhong在zai使shi用yong前qian一yi定ding要yao進jin行xing挑tiao選xuan及ji處chu理li,這zhe點dian非fei常chang重zhong要yao,也ye是shi廣guang大da食shi用yong菌jun栽zai培pei者zhe最zui易yi忽hu視shi的de問wen題ti。生sheng產chan實shi踐jian中zhong發fa現xian,發fa菌jun期qi間jian有you成cheng片pian菌jun棒bang兩liang頭tou接jie種zhong處chu有you雜za菌jun感gan染ran。這zhe種zhong情qing況kuang十shi有you八ba九jiu問wen題ti出chu在zai菌jun種zhong上shang。有you時shi在zai接jie種zhong過guo程cheng中zhong發fa現xian菌jun種zhong有you雜za菌jun,趕gan緊jin采cai取qu措cuo施shi也ye為wei時shi已yi晚wan。所suo以yi我wo們men要yao求qiu菌jun種zhong在zai使shi用yong前qian要yao有you專zhuan人ren認ren真zhen挑tiao選xuan。挑tiao選xuan人ren員yuan一yi定ding要yao有you多duo年nian菌jun種zhong生sheng產chan經jing驗yan,在zai光guang線xian較jiao好hao的de地di方fang進jin行xing。菌jun種zhong在zai使shi用yong前qian要yao進jin行xing處chu理li,因yin在zai菌jun種zhong培pei養yang過guo程cheng中zhong(大約30天),菌jun種zhong表biao麵mian會hui有you一yi層ceng灰hui塵chen,而er雜za菌jun孢bao子zi會hui附fu著zhe在zai灰hui塵chen上shang。一yi般ban來lai講jiang,對dui規gui模mo化hua生sheng產chan的de場chang家jia,培pei養yang室shi內nei雜za菌jun孢bao子zi濃nong度du較jiao一yi般ban房fang間jian高gao,特te別bie是shi用yong塑su料liao袋dai為wei菌jun種zhong容rong器qi,更geng是shi如ru此ci。因yin為wei菌jun種zhong袋dai的de破po口kou處chu很hen容rong易yi感gan染ran雜za菌jun,大da量liang雜za菌jun孢bao子zi會hui從cong破po口kou處chu飛fei散san到dao整zheng個ge菌jun種zhong培pei養yang室shi。處chu理li的de方fang法fa是shi:先用0.5-1%甲醛水洗去菌種表麵的灰塵,再將菌種浸入3%來蘇爾溶液中幾秒鍾撈出。
2.2.4 熏蒸消毒 :將滅菌後的菌棒搬運至接種室,再將空氣潔淨器、小板凳、接種用具、塑料周轉框(3-4個)、接種台、灑精燈、huochaidengfangrujiezhongshibiankekaishixunzhengxiaodu。yanwuxiaodujibeidianranhoukexunsuguanbimenchuangbingdakaiziwaixiandeng。tongshiyongyixiaojiezhongxiangjiangjiezhongfujinxingxunzhengxiaodu。jiezhongfuyaozhuanmendingzuo,bunengyongpingchangdebaidagua。
2.2.5 接種:當菌棒溫度降至26℃左右及接種室內煙霧消毒劑的氣味散盡時,接種人員在緩衝間洗手、換鞋、換帽、換衣服後可進入接種室內。先打開空氣潔淨器,然後3-4人一組開始接種。接種時點燃灑精燈,在其上方操作,動作要熟練快捷。一般是一人放種,2-3人解袋綁口。一瓶菌種(500ml)可接種菌棒15個(兩頭接種)。在接種過程中要禁止閑雜人員入內並盡量避免接種人員外出。此外接種人員盡量不要去拌料、發菌場所。生產實踐表明,接種人員應保持穩定,盡量不要換人。
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