高效液相色譜儀的常用數據處理方法

發布日期:2022-04-01
核心提示:高gao效xiao液ye相xiang色se譜pu儀yi的de儀yi器qi本ben身shen是shi記ji錄lu樣yang品pin通tong過guo檢jian測ce器qi時shi,吸xi光guang度du的de變bian化hua而er產chan生sheng的de電dian信xin號hao變bian化hua,從cong而er產chan生sheng色se譜pu譜pu圖tu,最zui終zhong的de結jie果guo就jiu要yao靠kao後hou期qi的de

高gao效xiao液ye相xiang色se譜pu儀yi的de儀yi器qi本ben身shen是shi記ji錄lu樣yang品pin通tong過guo檢jian測ce器qi時shi,吸xi光guang度du的de變bian化hua而er產chan生sheng的de電dian信xin號hao變bian化hua,從cong而er產chan生sheng色se譜pu譜pu圖tu,最zui終zhong的de結jie果guo就jiu要yao靠kao後hou期qi的de色譜數據處理係統。
譜pu圖tu處chu理li完wan即ji可ke製zhi作zuo校xiao正zheng曲qu線xian,校xiao正zheng曲qu線xian是shi數shu據ju處chu理li的de重zhong點dian,是shi將jiang標biao樣yang譜pu圖tu的de信xin息xi與yu數shu據ju做zuo成cheng一yi個ge標biao準zhun,供gong處chu理li試shi樣yang譜pu圖tu使shi用yong。校xiao正zheng是shi通tong過guo分fen析xi指zhi定ding的de準zhun備bei好hao的de標biao準zhun樣yang品pin,來lai確que定ding計ji算suan絕jue對dui組zu分fen濃nong度du的de響xiang應ying因yin子zi的de過guo程cheng。接jie下xia來lai介jie紹shao常chang用yong的de數shu據ju處chu理li方fang法fa:外(wai)標(biao)法(fa)是(shi)指(zhi)添(tian)加(jia)一(yi)定(ding)量(liang)的(de)標(biao)準(zhun)品(pin)於(yu)空(kong)白(bai)樣(yang)品(pin)中(zhong)製(zhi)成(cheng)對(dui)照(zhao)樣(yang)品(pin),與(yu)未(wei)知(zhi)檢(jian)測(ce)樣(yang)品(pin)平(ping)行(xing)進(jin)行(xing)樣(yang)品(pin)處(chu)理(li)並(bing)檢(jian)測(ce),根(gen)據(ju)對(dui)照(zhao)品(pin)響(xiang)應(ying)值(zhi)與(yu)標(biao)準(zhun)品(pin)的(de)濃(nong)度(du)函(han)數(shu)關(guan)係(xi)計(ji)算(suan)出(chu)未(wei)知(zhi)檢(jian)測(ce)樣(yang)品(pin)中(zhong)被(bei)測(ce)組(zu)分(fen)濃(nong)度(du)的(de)定(ding)量(liang)方(fang)法(fa)。
neibiaofazhideshijiangyidingzhongliangdechunwuzhizuoweineibiaowujiadaoyidingliangdebeiceyangpinhunhewuzhong,ranhouduihanyouneibiaowudeyangpinjinxingsepufenxi,fenbiecedingneibiaowuhedaicezufendefengmianjijixiangduixiaozhengyinzi,angongshijisuanchubeicezufendehanliang。內標法是把標準物質加入到被測樣品中,外標法不是把標準物質加入到被測樣品中,而是在與被測樣品相同的色譜條件下單獨測定。1、外wai標biao法fa要yao求qiu儀yi器qi重zhong複fu性xing很hen嚴yan格ge,適shi於yu大da量liang的de分fen析xi樣yang品pin,因yin為wei儀yi器qi隨sui著zhe使shi用yong會hui有you所suo變bian化hua,因yin此ci需xu要yao定ding期qi進jin行xing曲qu線xian校xiao正zheng。此ci法fa的de特te點dian是shi操cao作zuo簡jian單dan,計ji算suan方fang便bian,不bu需xu測ce量liang校xiao正zheng因yin子zi,適shi於yu自zi動dong分fen析xi。但dan儀yi器qi的de重zhong現xian性xing和he操cao作zuo條tiao件jian的de穩wen定ding性xing必bi須xu保bao證zheng,否fou則ze,會hui影ying響xiang實shi驗yan結jie果guo。2、內標法要求挺嚴格的,對於內標物的選擇要有一定的原則,適於分析樣品量較少的情況;不要求樣品裏的所有組分都出峰,隻要內標物和所關注的組分出峰並分離好就可以了;定量準確,對進樣量和操作條件的控製不很嚴格,但必須準確稱量試樣和內標物,否則會影響實驗結果·。
編輯:songjiajie2010