【總結】高效液相色譜儀的常用數據處理方法
發布日期：2022-09-15
核心提示：gaoxiaoyexiangsepuyideyiqibenshenshijiluyangpintongguojianceqishi，xiguangdudebianhuaerchanshengdedianxinhaobianhua，congerchanshengsepuputu，zuizhongdejieguojiuyaokaohouqidesepushujuchulixitong。
譜pu圖tu處chu理li完wan即ji可ke製zhi作zuo校xiao正zheng曲qu線xian，校xiao正zheng曲qu線xian是shi數shu據ju處chu理li的de重zhong點dian，是shi將jiang標biao樣yang譜pu圖tu的de信xin息xi與yu數shu據ju做zuo成cheng一yi個ge標biao準zhun，供gong處chu理li試shi樣yang譜pu圖tu使shi用yong。校xiao正zheng是shi通tong過guo分fen析xi指zhi定ding的de準zhun備bei好hao的de標biao準zhun樣yang品pin，來lai確que定ding計ji算suan絕jue對dui組zu分fen濃nong度du的de響xiang應ying因yin子zi的de過guo程cheng。接jie下xia來lai介jie紹shao常chang用yong的de數shu據ju處chu理li方fang法fa：外wai標biao法fa是shi指zhi添tian加jia一yi定ding量liang的de標biao準zhun品pin於yu空kong白bai樣yang品pin中zhong製zhi成cheng對dui照zhao樣yang品pin，與yu未wei知zhi檢jian測ce樣yang品pin平ping行xing進jin行xing樣yang品pin處chu理li並bing檢jian測ce，根gen據ju對dui照zhao品pin響xiang應ying值zhi與yu標biao準zhun品pin的de濃nong度du函han數shu關guan係xi計ji算suan出chu未wei知zhi檢jian測ce樣yang品pin中zhong被bei測ce組zu分fen濃nong度du的de定ding量liang方fang法fa。內nei標biao法fa指zhi的de是shi將jiang一yi定ding重zhong量liang的de純chun物wu質zhi作zuo為wei內nei標biao物wu加jia到dao一yi定ding量liang的de被bei測ce樣yang品pin混hun合he物wu中zhong，然ran後hou對dui含han有you內nei標biao物wu的de樣yang品pin進jin行xing色se譜pu分fen析xi，分fen別bie測ce定ding內nei標biao物wu和he待dai測ce組zu分fen的de峰feng麵mian積ji及ji相xiang對dui校xiao正zheng因yin子zi，按an公gong式shi計ji算suan出chu被bei測ce組zu分fen的de含han量liang。內標法是把標準物質加入到被測樣品中，外標法不是把標準物質加入到被測樣品中，而是在與被測樣品相同的色譜條件下單獨測定。1、waibiaofayaoqiuyiqizhongfuxinghenyange，shiyudaliangdefenxiyangpin，yinweiyiqisuizheshiyonghuiyousuobianhua，yincixuyaodingqijinxingquxianxiaozheng。cifadetedianshicaozuojiandan，jisuanfangbian，buxuceliangxiaozhengyinzi，shiyuzidongfenxi。danyiqidezhongxianxinghecaozuotiaojiandewendingxingbixubaozheng，fouze，huiyingxiangshiyanjieguo。2、內標法要求挺嚴格的，對於內標物的選擇要有一定的原則，適於分析樣品量較少的情況；不要求樣品裏的所有組分都出峰，隻要內標物和所關注的組分出峰並分離好就可以了；定量準確，對進樣量和操作條件的控製不很嚴格，但必須準確稱量試樣和內標物，否則會影響實驗結果・。
編輯：songjiajie2010
手機版
