保護柱的填料裝填方式也是選擇保護柱時要考慮的重要因素。目前有兩種填料方式：

 

　　（1）薄bo膜mo裝zhuang填tian法fa的de保bao護hu柱zhu，使shi用yong過guo程cheng很hen簡jian單dan方fang便bian，而er且qie可ke以yi在zai實shi驗yan室shi中zhong進jin行xing幹gan裝zhuang。但dan是shi，其qi最zui大da的de缺que點dian是shi不bu經jing濟ji，特te別bie是shi針zhen對dui中zhong國guo的de具ju體ti情qing況kuang，一yi次ci性xing使shi用yong相xiang對dui費fei用yong較jiao高gao。另ling外wai
，薄bo膜mo裝zhuang填tian法fa的de保bao護hu柱zhu所suo裝zhuang填tian的de色se譜pu填tian料liao有you限xian，隻zhi能neng提ti供gong很hen有you限xian的de保bao護hu作zuo用yong；不過也因為所裝填的色譜填料較少
，保護柱的長度也較短，所以對分析樣品的保留時間的影響也很小。

 

　　（2）還有一種保護柱結構其實質是縮短了的色譜分析柱，設計方式上有直連式、shoujinshihuozhengtishi。zhengtishishejishiyousepuzhudeshengchanchangshangzhijieanzhuangzaisepufenxizhushangde
，bixuyusepufenxizhuyitongdinghuo，keyifeichangfangbiandishiyong
，danbunenggoubeixiugai。zhilianshijiegoushejikezairenheshihouyousepugongzuozhelaianzhuanglianjie，keyiyurenhepinpaidesepufenxizhulianjieshiyong
，erqiehaikeyigenjuyangpindexiangguanqingkuangxuanzebutongdebaohuzhuchangdu。shoujinshibaohuzhucongjiegoushangjiangshiyuzhilianshibaohuzhuyiyang，zhishizailianjieshibuxuyaojiezhubanshoudenggongju，zhijieyongshoushangjinjike。lingwaicongbaohuzhujiegoudejiaodukan，baohuzhudejiegouyoufenweishifoukeyigenghuanbaohuzhuzhuxin。xianzaidaduoshudebaohuzhujunkeyigenghuanbaohuzhuzhuxin，keyijiangdibaohuzhudeshiyongchengben。daduoshurenshigenjusepufenxizhudetianliaoxuanzebaohuzhudetianliao，zhengchangqingkuangxiakeyixuanzeyufenxisepuzhuyiyangdeseputianliao。danshi，genjushijidefenxigongzuo，yekebubiyufenxisepuzhudetianliaowanquanxiangpipei。

 

　　總之，對於選擇保護柱的原則是：在滿足分析分離要求的前提條件下，盡可能地選擇較短的保護柱結構，盡可能選擇對分離樣品小一些保留性的填料。