(1)材料
 

瓊脂斜麵培養基
 

經分離培養的平板培養物。
 

(2)方法
 

①在瓊脂斜麵培養基試管上部標明移種的菌名(或編號)
、接種日期、接種者的組別及代號。
 

②zuoshouchichangyouxijundepingbandi，youshouchijiezhonghuan。jiezhonghuanzaihuoyanshangmiejunlengquehou，zhanqupingbanhuaxianshangfayulianghaodegulijunluo。bapingbanfanghuiyuanchu，lijiyongcishouquxiemianpeiyangjixiechi，yongyoushouxiaozhiyushouzhangjiachimiansai
，qingqingzhuandonghoubochumiansai，guankoutongguohuoyanmiejun。
 

③將已沾菌的接種環伸入斜麵培養基的管底部的凝固水，沿斜麵培養基的表麵從底向上劃一直線，然後再從底部向上劃連續曲折線；也可不劃直線隻劃曲折線。取出接種環
，管口通過火焰滅菌，塞好棉塞，放試管架上，接種環火焰滅菌後放下。
 

xiangxiemianpeiyangjishangyizhongdexijunruguoshishengchangzaixiemianpeiyangjishangde，huoshishengchangzaiyetipeiyangjizhongde，zeyongzuoshoutongshixiechijunzhongguanhexiemianpeiyangjilianggeguan，youshouwumingzhi、小xiao指zhi手shou掌zhang同tong時shi拔ba起qi夾jia持chi二er個ge棉mian塞sai。管guan口kou及ji接jie種zhong環huan先xian經jing火huo焰yan滅mie菌jun後hou，從cong菌jun種zhong管guan取qu菌jun立li即ji移yi種zhong到dao斜xie麵mian上shang，塞sai好hao棉mian塞sai，接jie種zhong環huan火huo焰yan滅mie菌jun後hou放fang下xia。然ran後hou將jiang斜xie麵mian培pei養yang基ji放fang37℃恒溫箱中培養24h。結果斜麵表麵形成菌苔。