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在zai製zhi備bei培pei養yang基ji時shi，應ying選xuan擇ze質zhi量liang符fu合he要yao求qiu的de脫tuo水shui培pei養yang基ji或huo單dan獨du配pei方fang組zu分fen進jin行xing配pei製zhi。脫tuo水shui培pei養yang基ji應ying附fu有you處chu方fang和he使shi用yong說shuo明ming，配pei製zhi時shi應ying按an使shi用yong說shuo明ming上shang的de要yao求qiu操cao作zuo以yi確que保bao培pei養yang基ji的de質zhi量liang符fu合he要yao求qiu，不bu得de使shi用yong結jie塊kuai、顏(yan)色(se)發(fa)生(sheng)變(bian)化(hua)或(huo)其(qi)他(ta)物(wu)理(li)性(xing)狀(zhuang)明(ming)顯(xian)改(gai)變(bian)的(de)脫(tuo)水(shui)培(pei)養(yang)基(ji)。對(dui)於(yu)已(yi)開(kai)封(feng)的(de)脫(tuo)水(shui)培(pei)養(yang)基(ji)應(ying)盡(jin)快(kuai)使(shi)用(yong)，如(ru)果(guo)不(bu)能(neng)及(ji)時(shi)用(yong)完(wan)，可(ke)以(yi)進(jin)行(xing)存(cun)放(fang)效(xiao)期(qi)驗(yan)證(zheng)，確(que)認(ren)最(zui)長(chang)存(cun)放(fang)周(zhou)期(qi)。脫(tuo)水(shui)培(pei)養(yang)基(ji)或(huo)單(dan)獨(du)配(pei)方(fang)組(zu)分(fen)應(ying)在(zai)適(shi)當(dang)的(de)條(tiao)件(jian)下(xia)貯(zhu)藏(zang)，如(ru)建(jian)議(yi)按(an)照(zhao)使(shi)用(yong)說(shuo)明(ming)書(shu)上(shang)的(de)要(yao)求(qiu)進(jin)行(xing)貯(zhu)藏(zang)，如(ru)低(di)溫(wen)、ganzaohebiguang，suoyouderongqiyingmifeng，youqishishengfangtuoshuipeiyangjiderongqi。weibaozhengpeiyangjizhiliangdewendingkekao，getuoshuipeiyangjihuogepeifangzufenyingzhunquechengliang，bingyaoqiuyouyidingdejingquedu。peizhipeiyangjizuichangyongderongjishichunhuashui，teshuqingkuangxia，kenengxuyaoyongqulizishuihezhengliushui。yingjilugechengliangwudezhongliangheshuideshiyongliang。
脫tuo水shui培pei養yang基ji應ying完wan全quan溶rong解jie於yu水shui中zhong，再zai分fen裝zhuang與yu滅mie菌jun。配pei製zhi時shi若ruo需xu要yao加jia熱re助zhu溶rong，應ying注zhu意yi不bu要yao過guo度du加jia熱re，以yi避bi免mian培pei養yang基ji顏yan色se變bian深shen。如ru需xu要yao添tian加jia其qi他ta組zu分fen時shi，加jia入ru後hou應ying充chong分fen混hun勻yun。應ying按an照zhao生sheng產chan商shang提ti供gong或huo使shi用yong者zhe驗yan證zheng的de參can數shu進jin行xing培pei養yang基ji的de滅mie菌jun。商shang品pin化hua的de預yu製zhi培pei養yang基ji必bi須xu附fu有you所suo用yong滅mie菌jun方fang法fa的de資zi料liao。培pei養yang基ji滅mie菌jun一yi般ban采cai用yong濕shi熱re滅mie菌jun技ji術shu，特te殊shu培pei養yang基ji可ke采cai用yong薄bo膜mo過guo濾lv除chu菌jun。用yong於yu環huan境jing監jian測ce的de培pei養yang基ji需xu特te別bie防fang護hu，以yi防fang止zhi外wai來lai汙wu染ran物wu的de影ying響xiang及ji避bi免mian出chu現xian假jia陽yang性xing結jie果guo，推tui薦jian用yong雙shuang層ceng包bao裝zhuang和he終zhong端duan滅mie菌jun。
培養基滅菌後不得貯藏在蒸汽滅菌櫃中，瓊脂培養基不得在0℃或0℃以下存放，因為冷凍可能破壞凝膠特性。培養基保存應防止水分流失，並在避光條件下。瓊脂平板最好現配現用，如置冰箱2~8℃保存，一般不超過1周，且應密閉包裝，若延長保存期限，保存期需經驗證確定。
固體培養基滅菌後的再融化隻允許1次，以避免因過度受熱造成培養基質量下降或微生物汙染。培養基的再融化一般采用水浴或流通蒸汽加熱。融化的培養基應置於45~50℃的環境中，不得超過8小時。傾注培養基時，應擦幹培養基容器外表麵的水分，避免容器外壁的水滴進入培養基中造成汙染。
