1、取鏡安放

shouchixianweijingdezhengquefangfayingshiyoushouwozhujingbi，zuoshoutuozhujingzuo。qiebukedanshouxieti，yifangmujinghuachu。shiyongxianweijingguanchashi
，xianweijingyingfangzaishentiqianfanglvepianzuodedifang
，yibianyongzuoyanguancha，youshouhuitu。　

2、對光 

轉動轉換器，使低倍物鏡對準通光孔。注意物鏡的前端與載物台要保持2厘(li)米(mi)的(de)距(ju)離(li)。雙(shuang)眼(yan)睜(zheng)開(kai)
，左(zuo)眼(yan)注(zhu)視(shi)目(mu)鏡(jing)
，將(jiang)遮(zhe)光(guang)器(qi)上(shang)較(jiao)大(da)的(de)光(guang)圈(quan)對(dui)準(zhun)通(tong)光(guang)孔(kong)
，轉(zhuan)動(dong)反(fan)光(guang)鏡(jing)，使(shi)光(guang)線(xian)通(tong)過(guo)通(tong)光(guang)孔(kong)反(fan)射(she)到(dao)鏡(jing)筒(tong)內(nei)。從(cong)目(mu)鏡(jing)中(zhong)就(jiu)可(ke)見(jian)到(dao)一(yi)個(ge)白(bai)亮(liang)的(de)圓(yuan)形(xing)視(shi)野(ye)。如(ru)光(guang)線(xian)過(guo)於(yu)強(qiang)烈(lie)，可(ke)調(tiao)小(xiao)光(guang)圈(quan)或(huo)用(yong)平(ping)麵(mian)反(fan)光(guang)鏡(jing)。

3
、壓片

壓片是將切片、塗tu片pian或huo裝zhuang片pian等deng玻bo片pian標biao本ben用yong金jin屬shu壓ya片pian夾jia固gu定ding在zai載zai物wu台tai上shang。壓ya片pian時shi，要yao使shi玻bo片pian上shang的de標biao本ben正zheng對dui通tong光guang孔kong的de中zhong心xin，標biao本ben小xiao時shi尤you其qi注zhu意yi這zhe一yi點dian。否fou則ze，標biao本ben會hui偏pian出chu視shi野ye，調tiao焦jiao時shi找zhao不bu到dao標biao本ben。　

4
、調焦觀察　　

用低倍物鏡guanchashi，bulunguanchahezhongbopianbiaoben，shouxianyingyongdibeiwujingguancha。dangduihaoguanghou，babopianbiaobenfangzaizaiwutaishang
，yongyapianjiayazhu，bingshibopianbiaobenzhongdebiaobenzhengduitongguangkongdezhongxin。ranhoushunshizhenzhuandongcuzhunjiaoluoxuan
，shijingtonghuanhuanxiajiang，zhidaowujingjiejinbopianbiaobenweizhi（一般距蓋玻片2―3mm）。鏡jing筒tong下xia降jiang時shi
，眼yan睛jing須xu從cong旁pang邊bian注zhu視shi物wu鏡jing
，以yi免mian物wu鏡jing碰peng到dao玻bo片pian標biao本ben

，壓ya碎sui蓋gai玻bo片pian，損sun壞huai鏡jing頭tou。然ran後hou左zuo眼yan向xiang目mu鏡jing內nei觀guan察cha，同tong時shi反fan方fang向xiang轉zhuan動dong粗cu準zhun焦jiao螺luo旋xuan，使shi鏡jing筒tong緩huan緩huan上shang升sheng，直zhi到dao對dui準zhun焦jiao點dian，看kan清qing物wu像xiang為wei止zhi。再zai轉zhuan動dong細xi準zhun焦jiao螺luo旋xuan，來lai回hui調tiao節jie，使shi看kan到dao的de物wu像xiang更geng加jia清qing晰xi。如ru果guo物wu像xiang不bu在zai視shi野ye中zhong間jian，則ze可ke一yi邊bian觀guan察cha，一yi邊bian用yong手shou移yi動dong玻bo片pian標biao本ben

，直zhi到dao所suo要yao觀guan察cha的de物wu像xiang進jin入ru視shi野ye中zhong間jian。值zhi得de注zhu意yi的de是shi，從cong目mu鏡jing中zhong看kan到dao的de物wu像xiang是shi倒dao像xiang。因yin此ci，玻bo片pian移yi動dong的de方fang向xiang與yu視shi野ye裏li物wu像xiang的de移yi動dong方fang向xiang正zheng好hao相xiang反fan。